ProPRIME utilizes a gentle cell lysis upon contact unlike existing paper matrix product(s) utilizing harsh extraction techniques and methods. ProPRIME solves the problem of protein denaturation during the lysis phase of a biological sample. Cell lysis is the disruption of the cell walls to release the biological components of the cell.

These include DNA, RNA, organelles, and lipids from the cell. Once the cell has lysed and spilled out its cellular contents, the cell has died. Cell lysis can occur naturally, in most cases through the drying process. It can also occur chemically and physically. Usually when a sample dries the cell and its components lyse onto a matrix or substrate. Cell lysis fractures the cell and releases its components entrapping the cellular contents within the matrix. As that occurs, the protein can denature or degrade and become essentially useless. Other commercially available matrix lysing products that instantly lyse cells are harsh and do not provide a suitable environment for the protein, hence denatures protein structures.

Primary protein structures are preserved. Proteins are the target with other cellular components (i.e. nucleic acids) trapped in the treated FP705™ paper as well. ProPRIME also inactivates proteins that cause the pathogens to be dangerous.

ProPRIME is proven to work with many current protocols. Applications with current paper matrix collection products using existing DNA processing techniques and protocols work with a slight modification or additional wash step. PRO is also amenable to automation for DNA analysis. Liquid handling systems can be utilized for protein or DNA extractions that include the silica-based extraction chemistries.

Samples

• Any specimen containing biological materials.

• Blood

• Buccal

• Semen

• Tissue

• Urine

• Plant Homogenate

• Cultured cells

• Plasmids

• Applications

• Protein / Nucleic Acid Assays

• Proteomics

• Protein Experiments

• Forensics

• Transgenic identification

• Transfusion medicine

• Plasmid screening

• Food and agriculture testing

• Drug discovery

• Genomics

• STR analysis

• Animal / Human identification

• Whole genome amplification

Benefits

• Easy Sample Collection

• Long term protein stabilization of primary structures

• Long term Nucleic Acids preservation

Efficient - Ready for processing in minutes

Storage - Ambient or Frozen